Where ever there is no drain carry the bucket to collect the water while flushing and avoid spillage of water on the floor. PROCEDURE: Sampling of water Sampling of water … Collect your water sample early in the week – Monday to Thursday –to give enough time to get to the laboratory and to be analyzed within 24-30 hours. Talk to the lab to determine if this collection procedure is acceptable for the analytical method they plan to perform. These Alert levels can be modified depending on the trend analysis done in the monitoring program. 4.11 Open the valve of sampling point slowly to full and drain water for approx. Annexure-3: Sampling Details of Purified Water. Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. Use sodium hypochlorite solution (1g/L free chlorine) or ethanol/isopropanol 70% v/v. Preparation of Containers for the sampling of purified water: Clean 1000 ml Clear glass bottles with a cap. Water system sanitization methods require assessment. %PDF-1.5 The sampling procedures and … If the hose is not connected but is provided, attach the hose using procedures applicable for that system. • There should be at least one sampling point directly after the clean-water drinking water samples and handling of sample preservatives. 3. Fill the bottle almost to the top, leaving a gap for mixing (approx 5 mm). 2 Content • Introduction – What does the future hold for microbiological water AQC? It is important to note that you don’t have to exceed specification in order to submit a result for identification, it’s a best practice to document and select and identify representative flora from the system. Water Sampling Procedure: 1. Write the sampling details or affix the label on the bottle as per Annexure-3. Purified water quality parameter trending shall be done on the Monthly basis, trend charts shall be reviewed and a conclusion shall be made (if required). <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Microbiological Analysis of Water Quality •Looking in to the 21st Century sigma-aldrich.com January 31, 2012 By Richard Glindon Presented by Klaus Herick. After completion of the analytical report, checked and approved by a designated person and after that generate the COA (if required). This document is not intended for public release. ISO 19458:2006 provides guidance on planning water sampling regimes, on sampling procedures for microbiological analysis and on transport, handling and storage of samples until analysis begins. Sampling … The final summary report shall be prepared at the end of the Year. for validated time. 2. Flush the sample port/valve for the qualified time or volume and immediately open the labeled sample container and collect the sample. Related: SOP for Corrective and Preventive Action (CAPA), List of SOP for Pharmaceutical Quality Assurance, SOP on Handling of Incidents and Deviations, SOP ON Receipt, Issue & Control Of Lab Chemicals, SOP for Qualification of Quality Control analyst, SOP On Safety In Quality Control Laboratory, SOP for Corrective and Preventive Action (CAPA), ← SOP For Handling of Power Failure in Plant, List of ICH Quality Guidelines for Pharmaceutical Industry, SOP List For Pharmaceutical Quality Assurance, Chemical TOC /Oxidisable Substance Total Microbial Counts Presence of Pathogens, Total microbial counts Presence of Pathogens. OBJECTIVE To lay down a procedure for microbiological monitoring of raw water and purified water SCOPE: This SOP shall be applicable for sampling and microbial analysis of raw and purified water from all user points. Noroviruses, are repeatedly related to poor water quality (… ^���\�L �&�BϘ�B���X�d&ǣ=�K�}. Fill the containers with 1000 ml and 125 ml or up to the neck of purified water for chemical and TOC analysis respectively, and close the lid immediately. Before analysis, Register/log the sample, and generate/issue the worksheet, or the template. Note: Before sampling, the aluminum foil covering the sampling point should be removed. Open the valve of the sampling point and allow the water to drain for about 20-30 seconds. Sampling water for subsequent analysis is often considered to be somewhat easier than sampling, say, soils for two main reasons: (i) water tends to be more homogeneous than soils, there is less point-to-point variability between two samples collected within the same vicinity, (ii) it is often physically easier to collect water samples because it can be done with pumps and hose lines, (iii) known volumes of water can … If sampling a body of running water, point the mouth of the bag upstream and your hands downstream to avoid contamination. for validated time. Sampling Instructions This method of sample collection is acceptable for EPA analytical methods 1622 and 1623 when sending water samples in to the laboratory without filtering in the field. It represents one aspect of water quality. Annexure-2: Sampling Schedule of Purified Water. Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. 3. 4. Microbiological samples should be collected in sterile plastic or glass bottles. Head QA shall be responsible for the approval of SOP. If the immediate analysis is not possible, store the sample under refrigeration at 2 to 8ºC. ACoP L8 recommends that sampling for Legionella should be carried out when: 2 0 obj Approximately add 15-20 ml of R2A / Plate count Agar into each Petri dishes. OBJECTIVE:To lay down a procedure for microbiological monitoring of raw water and purified water. Analyse the water samples for Microbiological analysis as per specifications. Carry the sampling containers to the sampling point as per the sampling schedule. Start microbiological analysis of water as soon as possible after collection to after sampling and certainly within 24 hours. Refer Annexure-2 for preparing the Sampling Schedule. If the following situation(s) is/are encountered, DO NOT TAKE SAMPLES. 4 0 obj 7`rV�XH���ق�*�r��θ �NMsp�^%B�+$��3�V�&�y�J09;��������;�[KeR)����XA_�ώ�z5]�Un6)���\��ٻ���O���T�E���\J0^5�4?���xE��� Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. If no sterile bottle is available, rinse the bottle first with hot water, then with the sample and then proceed to fill the bottle with sample. Flush water through sampling point for 5 – 20 Seconds. If the immediate analysis is not possible to acidify the sample with a 2-3 drop of 2.0 M HCL and store the sample under refrigeration at 2 to 8ºC. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. stream WATER ANALYSIS SAMPLING PROCEDURES Microbiological samples: Microbiological samples should be collected in sterile plastic or glass bottles which Vinlab supplies. Ensure that the required contact time for the sanitizer has been achieved. <> A sample volume of 200ml should be sufficient for Faecal coliform, E. coli and Heterotrophic plate count. Head of the Department / Designee of respective areas & QA shall be responsible for reviewing the SOPs. HOW TO COLLECT A WATER SAMPLE FOR MICROBIOLOGY EXAMINATION Created: March 26, 2015 Page 1 of 2 *Conditions Apply 1. Sampling personnel shall wear clean smocks or gowns and sterile gloves prior to collecting any sample (As per area requirements). Wrap the neck with aluminum foil to avoid any contamination during transportation. Rinse the container at least one to two times with purified water from the sampling point. – How can we meet the demands microbiological water AQC in the future? Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. Carry out the chemical analysis of samples as per the current version of purified water specification. Alert levels are specific for a given facility and are established on the basis of a baseline developed under an activity monitoring program. 4.2.2 Enter into the respective area and visually observe the machine or part of the machine. Collection of purified water for Microbiological analysis: Hold the sterile container near the sampling point. B 2. If any chemical parameter exceeds the limit, immediately inform the same QA, Production, and Engineering Department. 1 0 obj surroundings dusty, covered with debris, or poorly ventilated; 2. 5.1.4 For a sampling of Treated Raw water (May contain chlorine), add 0.1 ml of 5 % sodium thiosulphate solution in the bottles before sterilization. • Sampling points should be located in such a way that water can be sampled from reserve tanks and reservoirs, etc. It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. Sampling Procedures for Drinking Waters 1 PURPOSE This standard operating procedure outlines details for collection of drinking water samples for chemical, microbiological and radiological analysis. �d��5�ֹܵܯ�y!%r%��]iHg�3�g����U}�%�^u]}�l���Y{��������S}}����v��u���q�������""�trv���H2�O$F%�1�)���ϟe�5��ϟ�ϒ�o�ٿ�? Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis … Wipe any visible dust from the exterior of the sample port or valve using a lint-free wipe/cloth moistened with an approved sanitizer – do not spray or wipe the interior of the port or valve. Standard Operating Procedure (SOP) for sampling from different points and microbiological analysis/testing of Purified water samples in pharmaceutical plants. Good water quality is important in many settings, including those found for all drinking water systems, during food production and in fresh- and seawater for recreation and bathing activity. Prepare the sampling schedule (Tentative) by the first working day of every New Year. Wear gloves when collecting samples. Sampling Schedule of Purified Water: Step Three Remove Faucet Screen and 5.1.3 Samples for microbiological evaluation should be collected in Pre-sterilized screw cap glass bottles. Sanitize the hands with 70 % v/v IPA. Microbiologist shall wear sterilised gloves during the sampling activity. These stored samples should be analyzed within 6 hours. Sampling apparatus and containers shall be clean, sterile, and/or endotoxin-free as per test requirements. {cy�L���*3i��S��e�T�����4�VS�H�¤y��2��d�����4(�"^�xnfg���kS��p�'�T�@�ů�_�:�}��$w��>�&���=������o3!�J� �H�bZ� Analyze the TOC sample as quickly as possible on arrival at the laboratory. Analyst error should be rare. 3 0 obj SCOPE:This SOP shall provide the procedure for sampling and testing of raw water and purified water from all the user points and all the points across the critical functions in the water purification process. Then remove the sterile strip from the bottle. A designated person shall issue the template/worksheet. Vinlab supplies 100ml sterile plastic bottles. Chemical analysis: If you would like the entire volume, please send your email request for a zip file.. SUPPLY … endobj Analyze the sample of purified water for pathogens using filtration technique/Direct as per the current specification of Purified water. Annexure-1: List of Sampling Points of Purified Water. 4.1.16 Again collect the swab sample and repeat procedure following Point 4.1.1 to 4.1.13. A sample volume of 250 mL should be sufficient for Total coliform, Faecal coliform, E. coli and Heterotrophic plate count 4.13 Carry out sampling step 4.14 and 4.19 as rapidly as possible to avoid exposure of the sample to the external environment. Microbiological Sampling and Testing May 2019 Science Staff Office of Public Health Science 1. Utility system sampling requires additional care because samples are collected in controlled and uncontrolled areas of the facility. These guidelines collate and standardise PathWest’s microbiological analysis methods as part of the sampling, testing and analysis agreement between Western Australian Local Governments and the Department of Health. There is a need to identify the source of contamination. Immediate resampling of the specific location where the OOT/OOS occurred must be performed, to rapidly assess the condition of the system. Phase 2 requires a root cause analysis and corrective action. Please select the appropriate link in the table below to send your request via email, or contact Health Canada's publication office to make your request.. To lay down the procedure for Sampling and Testing of Purified Water. Domestic water systems are supplied with water fit to drink, so ACoP L8 advices that regular microbiological sampling is not required. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) RESPONSIBILITY Quality Control Executive / Officer ACCOUNTABILITY Quality Assurance Head. Carefully replace the cap. Rinse with purified water. Correct sampling procedures as instructed by international standard protocols must be adhered to; Routine microbiological testing of drinking water supplies, recreational waters, and environmental waters is essential for the protection of public health ; Programme of testing. 4.12 Control the valve of sampling point to avoid splashing. MICROBIOLOGICAL WATER SAMPLING - BASIC PROCEDURES Taking a Routine Bacteria Sample Properly Step One Do Not Rinse The Bottle The powder in the bottle is meant to be there and will not contaminate your sample. Water Sampling Procedure (with reference to ISO 5667 Part 5) (A) Assessment of Sampling Location Please take note of the conditions of the environment, sampling taps and pipes at the sampling location. If a hose is already connected to the port or valve, collect the sample directly from that hose. Open tap and run to waste, allowing removal of any excess solution. This Standard Operating Procedure is applicable to the Microbiology Department. Unsatisfactory environmental condition e.g. 6.0 PROCEDURE FOR ANALYSIS OF WATER SAMPLE: Carry out the determination under conditions designed to avoid extrinsic microbial contamination of the water sample for the analysis. 1 minute. Prepare a Sampling and Analysis Plan (SAP) which describes the sampling locations, numbers and types of samples to be For Microbiological Analysis: Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. 5.2.1 Pour Plate Method Dispense one ml of sample into two Petri dishes. Officer or Executive of the Microbiology department shall be responsible for the preparation of new or revision of existing SOP. If you are sampling more than one location you need a separate sample bottle for each location. Details of the type of sample containers and preservatives to be used are also included. Water quality — Sampling for microbiological analysis. It is impractical to attempt the routine isolation of pathogens because they are present in relatively small numbers compared with other types of micro-organism. PROCEDURE FOR MICROBIOLOGICAL MONITORING OF WATER. Ensure you have read the ‘Standard drinking water sampling procedure – microbiological’. SAMPLING FOR MICROBIOLOGICAL ANALYSIS In case of laboratory analysis for microbiological purpose, following guidelines are adopted to collect representative water samples: Sample Bottles Pre-sterilized sampling bottles of 200 ml capacity made of pre-sterilized disposible/autoclavable plastic, or good quality soda / borosilicate glass. Alert levels are always lower than Action levels. Microbial levels, specified in the standard operating procedure or specifications, when exceeded should result in an investigation to ensure that the process is still within control. Phase 1 focuses on the method and the analyst; the purpose of phase 1 is to verify that the data are valid. Analyze the sample as quickly as possible on arrival at the laboratory. Food Safety and Inspection Service: •Sampling methods •Assessing sampling plans and testing methods •Method validations and laboratory quality assurance •FSIS testing programs, methods and pathogen-specific issues to consider •Industry testing activities 2 Today’s Presentation. <> Phase 2 follows the determination of data validity and investigates the water system and all potentially affected lots of products. Perform the analysis as per the current version of purified water specification. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) It is important to routinely identify organisms recovered from water systems when: Waterborne organisms may be detrimental to finished products or processes in which the water is used. Moreover, there are many types of pathogen and each requires a unique microbiological isolation technique. These procedures should be utilized by drinking water service providers. Avoid unpredictable changes in the microbial population ii. Follow the procedures described below to assist in the collection of an acceptable sample and to maintain the integrity of the sample after collection. Aseptic Technique for Microbiological Testing. Wear gloves and nose mask before sampling. Step Two Wash Your Hands prior to taking the sample. After completion of the test, record the results in the datasheet and also enter the results in the respective software/module. PathWest Laboratory Medicine WA Manual: Water Examination Procedures Title: Collection And Transportation Instructions For Water Sampling Document Number: MWP119 Version Number: 1.7 Document Owner: Woodward, Robin Page 1 of 7 Date Issued: 14-Dec-2017 COLLECTION AND TRANSPORTATION INSTRUCTIONS FOR MICROBIOLOGICAL WATER SAMPLING 1. • In systems with more than one water source, the locations of the sampling points should take account of the number of inhabitants served by each source. x��[mo�6� �Aw�X_DIE�q�;�:M � Close the valve and bring the samples to the laboratory and make an entry in the purified water inward register. Water column sampling using dedicated sampler devices specially designed to assess the impact of offshore activities on sea water quality Groundwater sampling Collection of groundwater and analysis to international standards using a comprehensive range of equipment, including interface meters, low and high flow sampling pumps, flow through cells and calibrated field instrumentation 1. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. Rinse the bag twice with the sample water prior to filling and closing. %���� Quality Monitoring of Water for Pharmaceutical Use. endobj Close the container immediately and record the sampling time. make the schedule in such manner that generation point (For Chemical & Microbiology Analysis) & return loop (For Microbiology Analysis only) covered on a daily basis and other useful points (For Microbiology Analysis only) to be covered in rotation within a month. Analyze the sample of purified water for Total Viable Count using filtration technique as per the current specification of Purified Water. The changes may occur in the bacterial content of water on storage can be reduced to a minimum by ensuring that samples are not exposed to light and are kept cool. endobj The precautions taken to avoid contamination must be such that they do not affect any microorganisms that are to be revealed in the test (water analysis). Surface water sampling methods and analysis — technical appendices Standard operating procedures for water sampling- methods and analysis Looking after all our water needs Department of Water … If the Total bacterial count exceeds the Alert limit/Action limit, immediately inform the same to QA and Engineering personnel and investigation shall be done as per the SOP for “Handling of microbial Excursion in Water and Environment”. In water systems with inadequate quality control and sanitation, water could act as a vehicle for pathogenic microorganisms that originate from the faeces of wildlife including birds, livestock and pet animals, as well as humans. Wash 125 ml Amber glass or suitable bottles with a stopper with 2.0 M HCL or 2.0% nitric acid and rinse with purified water and dry the bottles at 105 ºC for at least one hour. <>>> Where potable (drinking water) water is stored, BS8558:2015 sets out a regime where 6 monthly microbiological activity is measured. Faecal contamination of water is routinely detected by microbiological analysis. W|b1*]�y.����>'O�ak�Oߜ�~8� ���9X�;�����#|������O�en�4�O# Hold the sterile container near the sampling point. 4.2 Rinse water sampling: 4.2.1 After cleaning, the Production Officer shall inform to QA Officer for the collection of the rinse water sample. If sampling from a water faucet, run the faucet for 1 minute before obtaining a sample. 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